International Symposium on Cereal Leaf Blights 2019 | University College Dublin, Ireland | 22-24 May 2019

High-throughput screening of new biopesticides and plant defence inducers on wheat against Zymoseptoria tritici


Remi platel*
Institut Charles Viollette (ICV-EA 7394), ISA, Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte dOpale, F-59000 Lille, France

Calonne M
Université catholique de Louvain, Earth and Life Institute, Mycology, Croix du Sud 2, box L7.05.06, B-1348 Louvain-la-Neuve, Belgium

Le Guenic S
Univ. Artois, UniLasalle, EA7519 – Unité Transformations & Agroressources, F-62408 Béthune, France

Lequart V
Univ. Artois, UniLasalle, EA7519 – Unité Transformations & Agroressources, F-62408 Béthune, France

Chaveriat L
Univ. Artois, UniLasalle, EA7519 – Unité Transformations & Agroressources, F-62408 Béthune, France

Joly N
Univ. Artois, UniLasalle, EA7519 – Unité Transformations & Agroressources, F-62408 Béthune, France

Omoboye O O
Ghent University, Lab. Phytopathology, Dept. Plants & Crops, B-9000 Ghent, Belgium

Ongena M
Microbial Processes and Interactions (MiPI), TERRA Teaching and Research Centre, Gembloux Agro-Biotech-University of Liege, B-5030 Gembloux.

Coutte F
Institut Charles Viollette (ICV-EA 7394), ISA, Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte dOpale, F-59000 Lille, France

Magnin-Robert M
Université Littoral Côte d’Opale, UCEIV - EA n°4492, Unité de Chimie Environnementale et Interactions sur le Vivant, SFR Condorcet FR CNRS 3417, CS 80699 – F-62228 Calais cedex, France

Randoux B
Université Littoral Côte d’Opale, UCEIV - EA n°4492, Unité de Chimie Environnementale et Interactions sur le Vivant, SFR Condorcet FR CNRS 3417, CS 80699 – F-62228 Calais cedex, France

Martin P
Univ. Artois, UniLasalle, EA7519 – Unité Transformations & Agroressources, F-62408 Béthune, France

Höfte M
Ghent University, Lab. Phytopathology, Dept. Plants & Crops, B-9000 Ghent, Belgium

Declerck S
Université catholique de Louvain, Earth and Life Institute, Mycology, Croix du Sud 2, box L7.05.06, B-1348 Louvain-la-Neuve, Belgium

Jacques P
Microbial Processes and Interactions (MiPI), TERRA Teaching and Research Centre, Gembloux Agro-Biotech-University of Liege, B-5030 Gembloux.

Halama P
Institut Charles Viollette (ICV-EA 7394), ISA, Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte dOpale, F-59000 Lille, France

Reignault Ph
Université Littoral Côte d’Opale, UCEIV - EA n°4492, Unité de Chimie Environnementale et Interactions sur le Vivant, SFR Condorcet FR CNRS 3417, CS 80699 – F-62228 Calais cedex, France

Siah A
Institut Charles Viollette (ICV-EA 7394), ISA, Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte dOpale, F-59000 Lille, France


Poster Presentation
Cultural Management, Fungicide Resistance and Epidemiology

Atrium, UCD O'Brien centre for Science
Poster 22

View this abstract online by visting isclb2019.com/see/ABS98658

In the current global context of chemical use reduction in agriculture, new alternative crop protection methods have to be developed. One of the most promising solution is the use of biocontrol products for the management of plant diseases. The goal of our work is therefore to identify new biocontrol compounds efficient on the wheat-Zymoseptoria tritici pathosystem and to characterize their modes of action. A high-throughput screening of a panel of more than 200 biomolecules or extracts of diverse origins and natures (bacterial and fungal extracts, glycolipids, lipopeptides, rhamnolipids, etc.) has been performed. Their direct antifungal activity has been evaluated using in vitro fungal culture and their indirect activity has been assessed on wheat plantlets by targeting chosen biomarkers involved in plant defence reactions against biotic stress. Preliminary results revealed that some biomolecules and extracts exhibit significant direct activity (total or partial fungal inhibition) that varies depending on the nature and the origin of each compound. Current investigations are in progress to determine minimal inhibitory concentration (MIC) and half-maximal inhibitory concentration (IC50) for each active compound. Furthermore, analyses of enzymatic assays focusing on key enzymes of defence pathways are performed to determine the potential defence activation by the compounds. The most active biomolecules or extracts (by taking into account both direct and indirect activities) will be selected for further analyses in order to characterize more precisely their modes of action.