International Symposium on Cereal Leaf Blights 2019 | University College Dublin, Ireland | 22-24 May 2019

Sensitivity of Algerian Pyrenophora teres population to QoI fungicidesas reveled by Pyrosequencing


Hamama-Iméne Lammari
Ecole Nationale Supérieure d’Agronomie, Département de Botanique, El-Harrach, Alger, Algleria. (2) Institut National de la Recherche Agronomique d’Algérie

Hamida Benslimane*
Ecole Nationale Supérieure d’Agronomie, Département de Botanique, El-Harrach, Alger, Algleria. (2) Institut National de la Recherche Agronomique d’Algérie

Alexandra Rehfus
(2) BASF SE, Agricultural Centre, Speyerer Strasse 2, 67117 Limburgerhof, Germany

Gerd Stammler
BASF SE, Agricultural Centre, Speyerer Strasse 2, 67117 Limburgerhof, Germany


Poster Presentation
Cultural Management, Fungicide Resistance and Epidemiology

Atrium, UCD O'Brien centre for Science
Poster 14

View this abstract online by visting isclb2019.com/see/ABS69519

Net blotch of barley caused by Pyrenophorateres(Died.)Drecshler, is currently one of the most destructive diseases on Barley (HordeumvulgaeL.) crops in Algeria, fungicides treatments are mainly used to its control. A total of 230 mono-conidial isolatesof this fungus were sampled in 2015/16 and 2016/17 seasons, from 17Algerian provinces, in 58 distinct geographical localities where barley is cultivated.I n order to identify fungicides resistant individuals,and prevent their extension in the pathogen population, isolates were assessed for resistance to Quinone outside inhibitors (QoI)fungicides. Resistance was screened using Pyrosequencing technology. F129L and G137R mitochondrial cytochrome b substitution associated with QoI resistancewere looked for. Our results showed that all tested isolates were identified as QoI-sensitive, since all possessed the F129L and G137R wild-type alleles. According to these, P. teres Algerian population is considered sensitive toward QoIs, which is reassuringfor farmers, and fungicides market in Algeria.This study is the first investigation related to mutations associated toQoI fungicides resistance in Algerian population ofP. teres.