International Symposium on Cereal Leaf Blights 2019 | University College Dublin, Ireland | 22-24 May 2019

Investigating the role of taxonomically restricted genes and their potentially antagonistic role in combatting secreted fungal effectors


Ciarán Brennan*
UCD School of Biology and Environmental Science and UCD Earth Institute, UCD O’Brien Centre for Science (East), University College Dublin, Belfield, Dublin 4, Ireland

Binbin Zhou
UCD School of Biology and Environmental Science and UCD Earth Institute, UCD O’Brien Centre for Science (East), University College Dublin, Belfield, Dublin 4, Ireland

Harriet R. Benbow
UCD School of Biology and Environmental Science and UCD Earth Institute, UCD O’Brien Centre for Science (East), University College Dublin, Belfield, Dublin 4, Ireland

Sobia Ajaz
UCD School of Biology and Environmental Science and UCD Earth Institute, UCD O’Brien Centre for Science (East), University College Dublin, Belfield, Dublin 4, Ireland

Sujit Jung Karki
UCD School of Biology and Environmental Science and UCD Earth Institute, UCD O’Brien Centre for Science (East), University College Dublin, Belfield, Dublin 4, Ireland

Gerard Hehir
Dept. Crop Science, Teagasc, Oak Park, Carlow, Ireland

Angela Feechan
UCD School of Biology and Environmental Science and UCD Earth Institute, UCD O’Brien Centre for Science (East), University College Dublin, Belfield, Dublin 4, Ireland

Ewen Mullins
Dept. Crop Science, Teagasc, Oak Park, Carlow, Ireland

Fiona Doohan
UCD School of Biology and Environmental Science and UCD Earth Institute, UCD O’Brien Centre for Science (East), University College Dublin, Belfield, Dublin 4, Ireland


Oral Presentation
Host-Pathogen Interactions

Moore Auditorium, UCD O'Brien centre for Science
23 May 2019, 12:20

View this abstract online by visting isclb2019.com/see/ABS61361

Effector triggered immunity and effector triggered defence are crucial in combatting pathogens specialised at infecting a particular host. In the case of the Wheat/Z. tritici interaction, a 10,000-year-old conflict has led to considerable armaments being developed on both sides. On the fungal side, effector proteins are secreted into the host capable of evading detection, supressing defences and altering responses are employed. In response to these threats, plant host receptors and small secreted proteins capable of detecting and countering these effectors are employed. A microarray focused on the latent phase response of a resistant (cv. Stigg) and susceptible (cv. Gallant) to Z. tritici infection was mined for taxonomically restricted genes (TRGs) within the Poaceae. From this analysis, we identified three TRGs that were significantly upregulated by Z. tritici. We silenced these three genes using virus induced gene silencing and observed an increase in susceptibility to STB in both cultivars. Most interesting was one gene, ‘TRG7’ that when silenced in cv. Stigg results in a breakdown of Stigg’s resistance. To test for interactions with secreted Z. tritici effectors, these TRGs were screened with a battery of small secreted proteins from Z. tritici using a yeast 2 hybrid system. Based on this screen, it appears that all three of these TRGs interact directly with at least one small secreted effector protein candidate of Z. tritici and could potentially be involved in combatting the secreted fungal effectors of Z. tritici.