International Symposium on Cereal Leaf Blights 2019 | University College Dublin, Ireland | 22-24 May 2019

Pangenome analysis of the wheat pathogen Zymoseptoria tritici from the UK


Eoghan Curran*
University College Dublin; School of Agriculture and Food Science

James Burke
University College Dublin; School of Agriculture and Food Science

Angela Feechan
University College Dublin; School of Agriculture and Food Science
UCD Earth Institute

Anna Tiley
University College Dublin; School of Agriculture and Food Science
UCD Earth Institute


Poster Presentation
Pathogen Functional Genetics and Genomics

Atrium, UCD O'Brien centre for Science
Poster 24

View this abstract online by visting isclb2019.com/see/ABS52932

The objectives of this study are to isolate Zymoseptoria tritici races, causal agent of Septoria tritici Blotch (STB), from infected wheat leaves and using DNA extracted from these races to construct a pangenome of Z. tritici in the UK. The material used in this study are derived from STB-infected wheat leaves from four locations across the UK. Infected leaves from 30-40 cultivars from each environment were collected in 2018 and will be collected again in 2019 and 2020. 15 isolates of Z. tritici are collected from each environment each year. These isolates make an environment specific inoculum which are then sprayed on blocks of five winter wheat cultivars at growth stage 39. Scoring occurs at 28 days post inoculation for percentage leaf area diseased. The inocula are sprayed on blocks individually or in mixes of two environments. The five cultivars are Andante, Exsept, Flame, Longbow, and Stigg.

 

The 2018 inoculation yielded the following scores from each inoculum or mixture: Swindon 14.6±8.8%; Bishop Burton 9.3±6.4%; Carnoustie 8.8±5.9%; Lenham 11.1±9%; Swindon and Bishop 12.2±22%; Carnoustie and Lenham 7.7±2.3%.

 

The Z. tritici isolates will be genotyped by next generation sequencing. The genomic information from the pathogen will be used to build a pangenome across the three years, allowing the examination of the overall UK Z. tritici pangenome, and how it is changing across space and time.

 

 

 

We would like to acknowledge Origin Enterprises-SFI for funding this research.