International Symposium on Cereal Leaf Blights 2019 | University College Dublin, Ireland | 22-24 May 2019

Genome wide association study of spot blotch at seedling and adult plant stages in India and Morocco


Sajid Rehman*
Biodiversity and Crop Improvement Program, International Center for Agricultural Research in the Dry Areas (ICARDA), Rabat, Morocco

Andrea Visioni
Biodiversity and Crop Improvement Program, International Center for Agricultural Research in the Dry Areas (ICARDA), Rabat, Morocco

Shyam Saran Vaish
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India

Shiv Pratab Singh
Department of Plant Pathology, Narendra Dev University of Agriculture and Technology, Kumarganj, Faizabad, Uttar Pradesh India

Siya Ram Vishwakarma
Department of Plant Pathology, Narendra Dev University of Agriculture and Technology, Kumarganj, Faizabad, Uttar Pradesh India

Sanjaya Gyawali
Biodiversity and Crop Improvement Program, International Center for Agricultural Research in the Dry Areas (ICARDA), Rabat, Morocco
Vegetable Seed Pathology Department, Washington State University, Mount Vernon, USA

Ayed M. Al-Abdallat
Department of Horticulture and Crop Science, Faculty of Agriculture, The University of Jordan, Amman 11942, Jordan

Ramesh Pal Singh Verma
Biodiversity and Crop Improvement Program, International Center for Agricultural Research in the Dry Areas (ICARDA), Rabat, Morocco


Oral Presentation
Host Genetics and Resistance Breeding

Moore Auditorium, UCD O'Brien centre for Science
24 May 2019, 11:20

View this abstract online by visting isclb2019.com/see/ABS43173

Barley spot blotch caused by Cochliobolus sativus (Bipolaris sorokiniana) is one of the major constrains to barley production worldwide in warmer regions. The study was undertaken to identify and estimate effects of loci underlying quantitative resistance to spot blotch at seedling and adult plant stages. A panel of 261 barley genotypes (HI-AM) consisting of released cultivars from North and South America, Europe, Australia, advanced breeding lines, and landraces from ICARDA, was screened for assessing resistance to spot blotch. Seedling resistance screening was conducted using two most virulent isolates from Morocco (ICSB and SB54) while adult plant resistance was assessed at two hot spot locations in India (Faizabad and Varanasi) under artificial inoculation using a mixture of virulent isolates prevalent in Uttar Pradesh (India). Both GLM and MLM model were employed in Tassel using principal component analysis and Kinship Matrix as covariates. Genome wide association mapping indicated a total of 23 QTL at seedling stage (14 for isolate ICSB and 9 for isolate SB54), while 15 QTL were detected for adult plant resistance (6 at Faizabad and 9 at Varanasi). Common QTL at seedling stage and adult plant were found across all barley chromosomes. QTL detected explained together the 73.24% of the variance for seedling resistance to isolate ICSB and 49.26% for isolate SB54. QTL for adult plant explained together 38.32% and 44.09% at Faizabad and Varanasi, respectively.  Several QTL identified in this study were also reported before in bi-parental and association mapping populations studies supporting our results. The promising QTL detected at both stages, once validated, can be used for MAS in spot blotch resistance breeding program globally.