International Symposium on Cereal Leaf Blights 2019 | University College Dublin, Ireland | 22-24 May 2019

Accelerating the cloning of resistance genes against Septoria tritici blotch


David Lopez
INRA/UCA UMR 1095 GDEC (Genetics, Diversity and Ecophysiology of Cereals), Clermont-Ferrand, France

Burkhard Steuernagel
John Innes Centre, Norwich, UK

Brande Wulff
John Innes Centre, Norwich, UK

Thierry Langin
INRA/UCA UMR 1095 GDEC (Genetics, Diversity and Ecophysiology of Cereals), Clermont-Ferrand, France

Olivier Robert
Florimond-Desprez, 3 rue Florimond-Desprez, BP 41, 59242, Cappelle-en-Pevele, France

Aurélie Evrard
Florimond-Desprez, 3 rue Florimond-Desprez, BP 41, 59242, Cappelle-en-Pevele, France

Cyrille Saintenac*
INRA/UCA UMR 1095 GDEC (Genetics, Diversity and Ecophysiology of Cereals), Clermont-Ferrand, France


Oral Presentation
Host Genetics and Resistance Breeding

Moore Auditorium, UCD O'Brien centre for Science
24 May 2019, 09:40

View this abstract online by visting isclb2019.com/see/ABS39011

Host resistance is one of the most sustainable ways to fight Septoria tritici blotch. However, a lack of cloned resistance genes limits breeding for resistant cultivars. Advanced genomic technologies have shaped the development of new approaches for rapid gene cloning. The “MutRenSeq” approach is a targeted resequencing method that was successfully developed for the discovery of plant disease resistance (R) genes encoding nucleotide binding and leucine-rich repeat (NLR) domains. However, not all genes conferring resistance to wheat diseases belong to the NLR family. Indeed, Stb6 and Stb16q, the only Septoria tritici blotch genes cloned thus far, encode receptor-like kinases (RLK). We therefore developed a strategy for targeted sequencing of this very large gene family. Using the most recent genomic resources from bread wheat and its relatives we identified ~45,000 RLK sequences corresponding to ~128 Mb, and developed a ~200,000 bait capture design. In a proof-of-concept experiment, we captured RLKs from seven EMS-derived loss-of-function mutants originating from the spring wheat Cadenza and quickly re-identified Stb6. This approach provides an opportunity to accelerate significantly the cloning of R genes encoded by RLKs to facilitate breeding for resistance to pathogens with apoplastic lifestyles, such as Zymoseptoria tritici or Stagonospora nodorum.